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Amosi Leonard Mbuji

Amosi Leonard Mbuji

Assistant Lecturer

College of Natural and Applied Sciences

Botany

Biography

My field of research includes  CRISPR-Cas9 technology, Integrated pest management, Insecticide resistance, Insect ecology, Insect rearing, Pest management, Applied entomology, Integrated crop management, Economic Entomology, Pesticide Toxicity, Agrochemicals, Storage Entomology, Insect Biotechnology, Forest entomology, Pesticide application technology, Insect pathology, Host plant resistance, Insect Physiology, Rearing, Insect Biochemistry, Biological Control, Biological Pest Control, Insect control, Insect pest management, Insecticide, Insect genome, Climate change, Insect bioassays, Life tables, Biopesticides, Resources utilization and Insect molecular biology.

Research Interest

Amosi Leonard Mbuji is an assistant lecturer and currently a PhD candidate in Agricultural Entomology and Pest Control at the College of Plant Protection, China Agricultural University (CAU) China. His field of research includes CRISPR-Cas9 Technology, Integrated pest management, Insecticide resistance, Insect ecology, Insect rearing, Pest management, Applied entomology, Integrated crop management, Economic Entomology, Pesticide Toxicity, Agrochemicals, Storage Entomology, Insect Biotechnology, Forest entomology, Pesticide application technology, Insect pathology, Host plant resistance, Insect Physiology, Rearing, Insect Biochemistry, Biological Control, Biological Pest Control, Insect control, Insect pest management, Insecticide, Insect genome, Insect bioassays, Life tables, Insect-climate climate change, Resources uses, Biopesticides, Resources utilization and Insect molecular biology

Contacts

Email:

Projects

Amosi Leonard Mbuji is an  entomologist whose work focuses on sustainable pest management and insect behavior, Environment conservation, crop protection and food security

 Key projects phased out and ongoing

  1. Eco-Friendly Pest Control in Tomato Cultivation
    Mbuji co-authored a study at Sokoine University of Agriculture in Tanzania that evaluated the effectiveness of botanical bio-pesticides specifically garlic, ginger, and castor oil extracts in controlling insect pests in tomato crops. The research identified whiteflies (Bemisia tabaci) and aphids (Aphis gossypii) as the most prevalent pests and demonstrated that these natural treatments significantly reduced pest populations and minimized yield loss by up to 25% .
  2. Cannibalistic Behavior in Fall Armyworm Larvae
    In collaboration with researchers in China, Mbuji investigated the cannibalistic tendencies of fall armyworm (Spodoptera frugiperda) larvae under laboratory conditions. The study found that older larvae exhibited increased predation on younger ones, especially at higher population densities. These findings have implications for optimizing mass-rearing practices and improving pest control strategies in agricultural settings .

  3. Insecticide Resistance and Fitness Costs 

    In a 2024 study published in Crop Protection, Mbuji and colleagues investigated the resistance development of H. armigera to tetraniliprole, a newly developed diamide insecticide. The research assessed the fitness costs associated with resistance, providing insights into the sustainability of using tetraniliprole for pest control

    4.Ongoing CRISPR Experiment on Helicoverpa armigera and Helicoverpa assulta

    Amosi Mbuji is a currently working on genetic engineering and molecular biology, with a focus on developing innovative pest control strategies through genome editing technologies. His work primarily targets the cotton bollworm (Helicoverpa armigera) and Tobacco budworm (Helicoverpa assulta), a significant agricultural pest known for its resistance to conventional insecticides.  I am targeting CYP6B6, CYP6B7 and CYP6B2 gene responsible for detoxification in the insects.

Publications

  1. Amosi Leonard Mbujia, Zhaoxiang Xuea, Mingcheng Guob, Mingjian Lia, Shenglan Lva, Lei Zhang. Resistance and fitness costs of the Helicoverpa armigera after selection with the tetraniliprole newly developed diamide insecticide. https://doi:10.1016/j.cropro.2024.106622. Publication Year: 2024. Journal of Crop protection.
  2. Mingjian Li, Amosi Leonard Mbuji, Chengxiao Zuo, Shenglan Lv, Xuegui Wang, Lei Zhang. Sub-lethal effects of broflanilide a novel meta-diamide on Spodoptera frugiperda after long exposure https://doi.org/10.21203/rs.3.rs-3367670/v1. Publication Year: 2024. Journal of Crop protection.
  3. Sheng-Lan Lv1, Zheng-Yi Xu1, Ming-Jian Li1, Amosi Leonard Mbuji1, Meng Gu1, Lei Zhang*, Xi-Wu Gao*. Detection of Chitin Synthase Mutations in Lufenuron-Resistant Spodoptera frugiperda in China-https://doi.org/10.3390/insects13100963. Publication Year:2023. Journal of Insect Science.
  4. Amosi Leonard MBUJI 1,2,3,# , Xueyan GONG 1,2,4,# , Fen LI 1,2,4 , Lei YANG 1,2 , Xueyan SHI 3, Zhengqiang Peng5, Ying ZHANG 3,* and Shaoying WU 1,2,4,*. A study on the cannibalism behaviour and predation of the larvae of fall armyworm (Spodoptera frugiperda)-  https://doi.org/10.1111/1748-5967.12584. Publication Year: 2022. Journal of Entomological Research.
  5. Amosi Leonard Mbuji1, * Amani Stephen Milinga 1, 2 Vincent Kiswaga. Environmentally friendly approach of controlling insect pests in tomato (Solanum lycopersicum L.) https://doi.org/10.7176/JEES. Publication Year:2022. Journal of Environment and Earth Science.
  6. Lei Yang1, Binglin Xing1 , Fen Li1 , Li Kui Wang1 , Linlin Yuan1, Amosi Leonard Mbuji1,2, Zhengqiang Peng3, Farag Malhat4 and Shaoying Wu1: Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes http://dx.doi.org/10.7717/peerj.12069. Publication Year:2021. Peer J